The speed of the centrifuge is the centrifugal acceleration. The magnitude of the centrifugal acceleration depends on the rotation speed of the rotor and the radius of rotation of the particles.
According to the speed classification, centrifuges can generally be divided into the following types:
Ordinary centrifuge (low speed) <8000r/min;
High speed centrifuge 8000 ~ 30000r / rain;
Ultracentrifuge 30,000 ~ 80000r / min;
Ultra high speed centrifuge >80000r/min.
Under normal circumstances, the medical centrifuge is a low-speed centrifuge, the speed is generally not more than 5000r / min, the horizontal rotor can be used, and can meet the needs of large capacity. Centrifuges used in schools, scientific research units, etc., generally require high-speed centrifuges (greater than 5000r/min) and small-angle angular rotors.
When selecting a centrifuge, the laboratory can select centrifuges with different capacities, different speeds and different temperature control according to the purpose of the experiment and the needs of the experiment.
For example, when it is required to be normal temperature, micro, and rapid centrifugation, a palm type centrifuge and a micro centrifuge can be used;
If the rotation speed does not exceed 8000r/min, for example, cells are collected during cell culture, an ordinary low-speed centrifuge can be selected;
If the required speed is greater than 12000 rpm, high-speed centrifuges can be used, such as small amount of plasmid DNA extraction, cDNA extraction experiments, protein extraction, competent preparation, and extraction of RNA from plants. The rotation speed needs 12000 rpm to 15000 rpm and the temperature is kept at 4 degrees. Environment, frozen high speed centrifuges can meet the requirements.
When purchasing a centrifuge, the user must first understand the purpose of the experiment and select the centrifuge of the appropriate speed according to the purpose of the experiment.
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