The new technology of three-dimensional cultivation of black fungus in large bags can not only solve the disadvantages of low yield and easy pollution of black fungus pouch cultivation, but also be free from climate conditions, sites, resources, funds, etc., saving labor, materials, yield and quality. , easy cultivation, short cycle, high benefits and so on.
First, the cultivation site
You can use vegetable greenhouses, free space, balconies, roofs, trees, trees and other places, but close to the water, ventilation, away from the source of pollution.
Second, the cultivation season
The best earing period is when the local temperature is stable at 15°C～25°C.
Third, raw material preparation
1. 90% cottonseed hull, 5% bran, 3% corn flour, 1% lime powder, 1% gypsum powder.
2. Cornstarch 80%, bran 10%, corn flour 5%, soybean powder 2%, quicklime powder 2%, gypsum powder 1%.
Fourth, bagging
The bacteria bag can choose 22 cm 38 cm 0.04 cm high-density polyethylene cylinder. When loading, it is necessary to try to hold tightly so that there is no gap between the bag and the material, and it is important to ensure that the water bag is formed and the cultivation fails.
Fifth, sterilization
Steam sterilization is generally used, and boilers of different specifications are selected according to the size of production. Household cultivation can be changed from steam drums to steam generators. At the time of restructuring, a mouth was cut on one side of the drum, and a steel pipe with a diameter of 2 cm was inserted from the opening into the other side of the drum, and the opening was welded tightly with the steel pipe. Open a 4 cm mouth at different locations on the same side of the opening, and use a 4 cm short steel tube (slightly inserted into the barrel wall) to tightly seal the opening. 2 cm pipe for water, 4 cm pipe for the exhaust pipe, with a brick into a stove, the bucket sitting on the stove, add water inside the bucket can be fired in the stove, so that the steam generated. In the middle of sterilization, water must be added frequently to avoid drying the barrel and affect the amount of steam generated.
VI. Inoculation
After the vaccination personnel enters the inoculation room, they can first scrub their hands with 75% alcohol cotton balls, and then each group of 3 people. One person digs out the bacteria from the bottle, and the two unpack the bags and tie the bags. Both ends of each bag can be inserted into 2 or 3 pieces of jujube size bacteria. All bacteria bags must be completely taken over at one time, and they must not be randomly accessed in the middle. Otherwise, they must be re-fumigated before reoperation.
Seven, bacteria management
After the bacteria bag is placed in a clean room or greenhouse to avoid light culture. Keep the temperature at 25 °C ~ 28 °C. Do not ventilate for the first 5 days. Increase or decrease the temperature in the shed by means of lightening or shading. When the light is increased, the bag stack must be shaded by a black film or grass curtain. After 5 days, it is suitable for proper ventilation and the humidity is kept below 70%. After 40 days to 50 days of culture, mycelium can be transferred to ear management after full bagging.
Eight, ear management
1. Site management. If the earing site is selected in the shed, a small band of 15 cm in width and 15 cm to 20 cm in height can be created in the shed. The distance between the soil spurs is 80 cm. Outdoors can also be used to make soil spurs such as hardening. The ground can be covered with the above distance.
2. Fungus bag opening. Once you have prepared the venue, you can cut the ear of the bag. The bag is first washed with 1% potassium permanganate solution or 0.2% clotrimazole solution, and then the surface of the bag is cut with 4 to 6 "V"-shaped ports at both ends with a sharp blade. The diameter is about 2 cm.
3. Put bacteria bags. Put the bacterium bag flat on the soil squatting after the hatching. The distance between the bag and the bag is 5 cm to 8 cm. The soil is filled with soil in the middle. If it is hardened, it can be filled without filling the soil. Centimeter - 3 cm thick soil layer, and then according to the first pendulum method set the second layer of bacteria bags. If you do not fill the soil, put two small bamboo poles with a diameter of 2 cm to 3 cm on the first bag, and then put a second layer of bacteria bags. In this way, 6 layers to 8 layers are placed in order. Finally, a layer of mulch film was placed near the mound to prevent the soil particles from splashing on the ear during water spraying.
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