The straw fermentation live dried bacteria are composed of lignocellulose decomposing bacteria and organic acid fermenting bacteria. In order to grasp the growth and decrement of straw drying live bacteria in the process of straw micro-storage, we determined the live fermentation of straw during the micro-storage of wheat straw. Biochemical changes.
1 Materials and methods
1.1 Straw fermentation live dried bacteria: Straw fermentation live dried bacteria are provided by the Xinjiang Star Farm Technology Extension Service Station in Urumqi.
1.2 Production of wheat straw micro-storage: According to the method of literature 1.
1.3 Counting of lignocellulose decomposing bacteria and organic acid fermenting bacteria: According to the method of literature 2-6.
1.4 Determination of C 1 enzyme and C x enzyme: According to the method of literature 7.
1.5 Storage pH Determination of organic acids: According to the method of literature 8.
2 Results and Analysis
2.1 Results: The change of wheat straw and cellulase activity from the start of microbiological storage to 28 days, and the results of the determination of the results are shown in the table below.
2 . 2 Analysis
(1) Growth and growth of straw-dried live dried bacteria: From the beginning of the micro-grain storage to the 7th day, the straw fermentation live-dried bacteria are in the logarithmic growth phase, in which the lignocellulose decomposing bacteria proliferate 150-fold, and the organic-acid-fermenting bacteria proliferate 90-fold. , and then enter the stable growth stage.
Counting results on the 21st day showed that the total number of living cells of the lignocellulose-degrading bacteria was reduced by 6.7%, but microscopic examination revealed that 85.5% of the cells formed spores or spores. The total number of living cells of the organic acid fermentation bacterium was reduced by 38.9 %. On the 28th day of fermentation, microscopic examination revealed that all intrinsic cell-decomposing bacteria formed spores or spores. The total number of viable cells was still the same as on day 21, and no organic acid-producing bacteria were detected at a dilution of 108.
(2) Changes in cellulase activity: During the logarithmic growth phase of live dry straw cultures, C 1 enzyme activity and C x enzyme activity were increased by 150-fold and 31.6-fold, respectively, and then gradually decreased. Compared with the 7th day, the C1 enzyme activity on day 21 dropped by 40%, and the Cx enzyme activity decreased by 43.1%. On the 28th day of fermentation, the C1 enzyme and the Cx enzyme were all inactivated.
3 Discussion The concentration of organic acids in the storage material gradually increased with the extension of the micro-storage time, and the pH value gradually decreased with the increase of the organic acid concentration. Due to the increase of organic acid concentration and the decrease of pH, cellulase activity was gradually inhibited, but C 1 enzyme and C x enzyme have higher activity in the environment of pH 4.8 - 6.2, indicating that the performance of low pH resistance is better. it is good.
When ruminants feed on straw micro-feeds, the lignocellulosic decomposing bacteria enter the rumen with micro-storage in the form of spores or spores. The activity of lignocellulose-degrading bacteria in the rumen remains to be further studied. (October 1993)
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