The gold algae have a small cell size and complete nutrition, making them ideal for bivalve larvae. G. algae has high requirements for ecological conditions, and is particularly vulnerable to contamination. It is difficult to cultivate a large number of species. In the past few years, the authors used a fully enclosed plastic film bag (shrimp bag) to cultivate echinoid algae. The Zhanjiang fork-side gold algae reached the level of daily supply of 2t, which greatly improved the survival rate of loach larvae and gave satisfactory results. The training techniques are introduced as follows:
First, the selection of the original species quality of the original species has a great influence on the culture results, so when selecting the original species requires the selection of pollution of invincible pests, strong vitality, strong growth, normal color of the algae without a large number of precipitation and no significant attachment to the wall The algae species. In general production units, due to the strong seasonality in production, poor facilities, equipment, and instruments, and relatively weak technical forces, it is difficult to maintain the above-mentioned requirements for perennial conservation. Usually purchase the original species from the relevant scientific research institute, college or conservation center before the production season. After the primary species is retrieved, secondary expansion culture is conducted. After a certain amount is reached, tertiary productive culture is performed.
The purpose of the second and third level of productive culture is to expand the training and meet the requirements for quality and quantity required for production.
1. After the seawater treated seawater was precipitated in the reservoir for 24 hours and Erdaosha was filtered, 25mg/L of bleaching powder was added for disinfection. Kill harm. After 12 hours (usually before the next morning inoculation), the disinfecting water was pumped from the disinfecting tank to another water tank with a submersible pump. After aeration, the residual chlorine was removed, and then 5-10 mg/L of sodium thiosulfate was added. with.
2. Disinfection of utensils before vaccination In order to prevent bio-fouling in the air, vaccination should be carried out indoors.
1 The vaccinated personnel shall be replaced with ultraviolet-disinfected special clothes before entering the inoculation room, and the operated hands shall be sterilized by soaking in 20 mg/L potassium permanganate solution.
2 All utensils and plastic film bags should be soaked in 2Omg/L potassium permanganate solution for 5 minutes before being used after being washed with disinfected seawater.
3 According to the requirements for inoculation, the neutralized and disinfected seawater is pumped into a container (a plastic bucket with a capacity of about 25 OL) and nutrient salt is added. The iron salt in nutrients dissolves slowly and is used in small quantities. It can be formulated into mother liquor several days in advance; other nutrients can be weighed according to the formula of the culture solution and then dissolved in cold water or pure drinking water. Disinfected seawater is used immediately after stirring. The formulation of the culture solution is as follows:
CON2H4 4Omg
NaNO3 35mg
KH2PO4 5mg
FeC6H5O7 0.2mL (1% solution)
Vitamin B1 1O0μg
Vitamin B12 5μg
Seawater 1000mL
4 The proportion of vaccination is generally 1:4. If the climatic conditions are more appropriate, it can be inoculated in a ratio of about 1:6.
5 The inoculated plastic film bag is usually a thin double-layer shrimp seedling bag with a capacity of 75L, which is easy to handle and not easily damaged. Each bag can be 25L, the rest as full of air, the bag mouth with a good elastic rubber banding seal.
6 When inoculating, you usually need 5 people, one person to take algae species, one person to install a new culture solution, two people supporting the film bag mouth, one person tying a sealed bag mouth.
7 The inoculation time should be selected between 8:00-10:00 am. Try to make the temperature difference between the algal fluid of the algae species and the new culture fluid similar to avoid precipitation.
3. After the management vaccination is completed, the semi-pace (bottom close to the ground to prevent dumping) method will be stacked on the indoor floor with white tiles, one row per 2m, and 4 bags per square meter. Pay attention to the walkway about 6m away from each team when hanging and stacking to facilitate management and observation. Operation and so on.
two. Observe daily observation of growth. According to the change of color and the occurrence of air bubbles, it can be judged whether the growth is normal or not. Usually, the color of the second day begins to inoculate, the color is slightly darker, and small bubbles are generated to indicate normal growth; in the third and fourth days, the color turns deeper. A large number of air bubbles adhered to the wall, indicating strong growth; the darkest days of the fifth and sixth days were darker than the fourth day, indicating that the growth peaked and the counting concentration was about 300 cells/mL, which could be fed at this time; Going down gradually began to decline, precipitation. Therefore, feeding should be controlled on days 6-7 after inoculation.
2. Mix 4-5 times a day for regular swings.
3. Illumination adjustment Illumination is suitable or not, and has a great relationship with the growth of algae. As the production unit mainly uses natural light sources, full manual control is difficult. In the strong light of the afternoon, use a white cloth Dacron curtain, the window light for > 4 hours, to prevent direct light irradiation.
Fourth, several issues that must be noted
1. In management, new bags should be replaced in time if they are found damaged or leaked.
2. If there is no color change and bubbles generated within two days after inoculation, and there is a clear color difference in the same batch, it means that the bag inoculation fails and should be promptly given up and cleaned up.
3. For each feeding, a certain amount of algae with good color, strong vigor, and no precipitation should be reserved as the original seed for the next inoculation.
4. The date of inoculation of each batch of bait should be as close as possible to the date required for seedling production. Otherwise, human and material resources will be wasted, and even the failure of nursery will be caused.
First, the selection of the original species quality of the original species has a great influence on the culture results, so when selecting the original species requires the selection of pollution of invincible pests, strong vitality, strong growth, normal color of the algae without a large number of precipitation and no significant attachment to the wall The algae species. In general production units, due to the strong seasonality in production, poor facilities, equipment, and instruments, and relatively weak technical forces, it is difficult to maintain the above-mentioned requirements for perennial conservation. Usually purchase the original species from the relevant scientific research institute, college or conservation center before the production season. After the primary species is retrieved, secondary expansion culture is conducted. After a certain amount is reached, tertiary productive culture is performed.
The purpose of the second and third level of productive culture is to expand the training and meet the requirements for quality and quantity required for production.
1. After the seawater treated seawater was precipitated in the reservoir for 24 hours and Erdaosha was filtered, 25mg/L of bleaching powder was added for disinfection. Kill harm. After 12 hours (usually before the next morning inoculation), the disinfecting water was pumped from the disinfecting tank to another water tank with a submersible pump. After aeration, the residual chlorine was removed, and then 5-10 mg/L of sodium thiosulfate was added. with.
2. Disinfection of utensils before vaccination In order to prevent bio-fouling in the air, vaccination should be carried out indoors.
1 The vaccinated personnel shall be replaced with ultraviolet-disinfected special clothes before entering the inoculation room, and the operated hands shall be sterilized by soaking in 20 mg/L potassium permanganate solution.
2 All utensils and plastic film bags should be soaked in 2Omg/L potassium permanganate solution for 5 minutes before being used after being washed with disinfected seawater.
3 According to the requirements for inoculation, the neutralized and disinfected seawater is pumped into a container (a plastic bucket with a capacity of about 25 OL) and nutrient salt is added. The iron salt in nutrients dissolves slowly and is used in small quantities. It can be formulated into mother liquor several days in advance; other nutrients can be weighed according to the formula of the culture solution and then dissolved in cold water or pure drinking water. Disinfected seawater is used immediately after stirring. The formulation of the culture solution is as follows:
CON2H4 4Omg
NaNO3 35mg
KH2PO4 5mg
FeC6H5O7 0.2mL (1% solution)
Vitamin B1 1O0μg
Vitamin B12 5μg
Seawater 1000mL
4 The proportion of vaccination is generally 1:4. If the climatic conditions are more appropriate, it can be inoculated in a ratio of about 1:6.
5 The inoculated plastic film bag is usually a thin double-layer shrimp seedling bag with a capacity of 75L, which is easy to handle and not easily damaged. Each bag can be 25L, the rest as full of air, the bag mouth with a good elastic rubber banding seal.
6 When inoculating, you usually need 5 people, one person to take algae species, one person to install a new culture solution, two people supporting the film bag mouth, one person tying a sealed bag mouth.
7 The inoculation time should be selected between 8:00-10:00 am. Try to make the temperature difference between the algal fluid of the algae species and the new culture fluid similar to avoid precipitation.
3. After the management vaccination is completed, the semi-pace (bottom close to the ground to prevent dumping) method will be stacked on the indoor floor with white tiles, one row per 2m, and 4 bags per square meter. Pay attention to the walkway about 6m away from each team when hanging and stacking to facilitate management and observation. Operation and so on.
two. Observe daily observation of growth. According to the change of color and the occurrence of air bubbles, it can be judged whether the growth is normal or not. Usually, the color of the second day begins to inoculate, the color is slightly darker, and small bubbles are generated to indicate normal growth; in the third and fourth days, the color turns deeper. A large number of air bubbles adhered to the wall, indicating strong growth; the darkest days of the fifth and sixth days were darker than the fourth day, indicating that the growth peaked and the counting concentration was about 300 cells/mL, which could be fed at this time; Going down gradually began to decline, precipitation. Therefore, feeding should be controlled on days 6-7 after inoculation.
2. Mix 4-5 times a day for regular swings.
3. Illumination adjustment Illumination is suitable or not, and has a great relationship with the growth of algae. As the production unit mainly uses natural light sources, full manual control is difficult. In the strong light of the afternoon, use a white cloth Dacron curtain, the window light for > 4 hours, to prevent direct light irradiation.
Fourth, several issues that must be noted
1. In management, new bags should be replaced in time if they are found damaged or leaked.
2. If there is no color change and bubbles generated within two days after inoculation, and there is a clear color difference in the same batch, it means that the bag inoculation fails and should be promptly given up and cleaned up.
3. For each feeding, a certain amount of algae with good color, strong vigor, and no precipitation should be reserved as the original seed for the next inoculation.
4. The date of inoculation of each batch of bait should be as close as possible to the date required for seedling production. Otherwise, human and material resources will be wasted, and even the failure of nursery will be caused.