The technique of Efficient & Rapid Non-test Tube Plant Cloning is praised by the domestic and foreign media as the “Master of Green Copying†---- the research and development of the new technology pioneered by Li Changkai at home and abroad. . Li Changwei is currently the executive director of the China High-tech Industrialization Research Association, the Standing Committee Member of the High-tech Professional Committee of the China Association of Agricultural Technology Promotion, the Standing Director of the China Quality Inspection Association, the Chairman of Ningxia Kelvin Industrial Co., and the Director of the Ningxia Cologne Institute for Biological Engineering Development. . He has published more than 30 research papers such as "The Plant Non-tube-efficient Rapid Propagation Technology". He was the scientist engaged in the research and development of plant biotechnology earlier in the country; he first proposed the concept of “promoting rapid propagation as a leading industry and a high-efficiency industry†at home and abroad, and was a famous person who advocated and personally practiced the “rapid propagation industryâ€. Technology entrepreneurs.
The "plant non-tube efficient rapid propagation technology (TERNPC)" invented by the researcher Li Changzhen is a brand-new plant rapid propagation system. This technology has been proved through long-term application practice, it also has two major breakthroughs in modern plant biotechnology in plant tissue culture test tube rapid propagation and human history application of nearly 2000 years of conventional seedling raising technology.
This technology is a proprietary technology that has been successful in the rapid propagation of nearly 700 kinds of economic plants, showing that it is of universal significance to the rapid propagation of most economic plants. The plant non-in vitro proliferation technology was newly confirmed by the 1995 patent disclosure (announcement) patent document search and the Chinese Academy of Agricultural Sciences Documentation and Information Center, which is an internationally advanced level. The plant non-tube high-efficient rapid propagation technology (TERNPC) intangible assets were evaluated by Beijing's authoritative asset assessment agency as RMB 1.604 billion in October 1999 by the Ministry of Finance, setting the highest intangible assets of China's individual agriculture-related technology. In 2000, it was searched and searched by the Sichuan Institute of Science and Technology. The technology still ranks among the leading international standards. In April 2002, the search center of the Institute of Scientific and Technical Information of Shanxi Province confirmed the new confirmation. In April 2002, it was searched and confirmed by the database of the National Science and Technology Innovation Consulting Unit - Hunan Institute of Science and Technology Information. So far, no other organizations and individuals have done so. The exact same technical study. On April 30, 2002, it was assessed by an authoritative asset assessment agency that the technology's intangible assets reached 1.628 billion yuan.
This technology has won the first China Academy of Agricultural Sciences Technology Fair Golden Award, the ninth China Invention Exhibition Gold Award, Yinchuan City Science and Technology Progress Award. In 1995, Yinchuan City was listed as a 95 key high-tech development plan; in 1996 and 99, it was listed as a key scientific and technological achievement promotion project of the autonomous region by the Ningxia Science and Technology Commission, and the National Science and Technology Commission was included in the national-level scientific and technological achievements promotion program; The Party Committee Outcome Office and the Association of Rural Professional Technology of the China Association for Science and Technology have separately issued a document to promote this technology nationwide; in 2000, Sichuan Province was listed as a Torch Program project; in 2001, it was listed as a national high-tech industrialization promotion project by the State Planning Commission.
Rapid propagation of plants is a matter of great concern to people of all ages. In 1902, German famous botanist Haberlandt proposed that plant cells have pluripotency and could be cultivated in vitro to become a complete plant, guiding the numerous Chinese and foreign researchers to begin climbing new scientific peaks. In 1958, fcsteward used carrot root cells to cultivate complete plants, flowering and fruitful, and achieved major breakthroughs, laying an important foundation for plant tissue culture technology procedures. This has brought about important advances in a wide range of disciplines including plant morphology, cells, physiology, biochemistry, genetics, asexual rapid propagation, breeding, agriculture, forestry, horticulture, and medicine. At home and abroad have been used for the rapid propagation of high-value flowers, fruit trees, vegetables, oil, landscaping seedlings, food crops and valuable Chinese herbal medicines, and rapid propagation of some seedlings has entered the stage of factory production. However, over the past 40 years, vegetative propagation seedlings that are most urgently needed, large, and low-cost in production have been faced with a one-time investment in plant tissue culture, high costs, and complicated technical steps. Poor susceptibility, farmers can not be directly used in production, plant survival rate of test tube clones is low, difficult to promote and other shortcomings, the test tube cloning technology in the actual rapid propagation of seedlings in the production of the productivity is still quite limited, the real formation of a large-scale industry The number of plant species in the world does not exceed 100. Up to now, there have been many achievements in the laboratory, and most of the production techniques of fast-producing varieties are still in the hands of scientists. Few projects have truly achieved industrialization goals. Although innumerable scientists have made arduous and remarkable efforts to reduce costs, simplify processes, and increase propagation coefficients for decades, people still cannot make plant tissue culture techniques for many rapid propagation of woody plants and tissue culture of conifers ( Tissue culture translates into enormous productivity on a large scale. Scientists in China have made great efforts to use plant tissue culture technology. Many plants have been successfully researched and can be rapidly propagated. However, only about 10 kinds of economic plants form a production line and are popularized in large areas, and the development speed is relatively slow. Traditional nursery techniques such as cuttings, grafting, layering, and ramets have been used for nearly 2000 years in human history. They are still being used at home and abroad. As a method of asexual propagation, the number of seedlings currently produced worldwide using this type of technology is the largest. The reason is that it has a long history, simple technology, the largest number of people engaged in production, and easy promotion. Its advantages also determine its long service life. However, it suffers from slow propagation, low production generation per year, and can not be industrialized for continuous production. It has not been resolved for a long time.
The plant non-tube high-efficiency rapid propagation technology (TERNPC) uses many plant-like microincubation material units (including one leaf and one bud) of 3 mm to 1 cm in length cultivated in the stem and apices of the test tube, and is directly inoculated with the aid of simple conditions. On the Datian sand bed, most of the economic plant explants were regenerated into complete plants 4 to 11 days after the second generation, and the survival rate was as high as 85% to 100%. Progeny breeds every 15 to 60 days and can be quickly and efficiently reproduced by geometric progression. The propagation coefficient is above 2-15, which is higher than the rapid propagation coefficient of many plants in vitro. Under the condition of completely leaving the plant tissue and cultivating various complicated equipments, a variety of plants can achieve efficient and rapid propagation under test tube conditions. The plant non-tube high-efficiency rapid propagation technology (TERNPC) has undergone continuous development, production and promotion of various climatic zones and various soils throughout the country for 20 years. It has formed a complete technical system and is a very mature technology. Due to adopting a series of effective standardized technical measures and management experience, hundreds of thousands of specially trained general staff can be directly implemented in the field under simple conditions, which enhances the susceptibility and popularity of rapid propagation technology. The actual comprehensive rapid propagation efficiency is enhanced, and the production cost of factory plant nursery is minimized. One-time fixed investment is dozens of times lower than tissue culture rapid propagation technology. This technology perfectly and wisely overcomes all the shortcomings of plant tissue culture tube propagation and conventional nursery techniques, and retains and exploits all the advantages of both of them. When reaching a certain scale above the nursery, the investment ratio of production can reach 1:5--1:10. It is very economizing on plant germplasm materials, and it only begins to multiply for most plants using 3 mm-1 cm long micropropagative material units (including one leaf and one bud), and can produce hundreds of thousands or even tens of millions in continuous production for one year. Purebred clone seedlings.
The advanced nature of plant non-tube high-efficiency rapid propagation technology (TERNPC) compared with plant tissue culture and traditional nursery techniques, and its characteristics in technical production and use are as follows:
1. The use of tiny explants with a length of 0.3-1.0 cm as a breeding unit material greatly saves the germplasm material. The amount of material used for explant regeneration is 3-8 times less than conventional seedlings; the inoculation rate is extremely high. Fast, 3-5 times that of organizational culture. Directly inoculated in Datian sand bed or nutrient bag, once seedling until supply, without any movement, the survival rate is high. With complete departure from the tissue culture building and the rapid propagation of all test tubes, the nursery facilities are several ten times lower than the tissue culture rapid propagation and lower than the conventional nursery.
2. Under the original and simple conditions, regardless of the northern and southern parts of the country, at different latitudes, in different soils, and in different climates, this method can be used for rapid propagation throughout the year (including extreme temperatures: low temperature -35 degrees and high temperature 42 degrees), and most varieties are Up to 6-12 generations. This technique is less affected by seasons and can be inoculated and propagated 365 days a year. Each generation is efficiently proliferated in a geometric progression based on the original plant species. This rapid propagation technology can be used to start production on any day of the year. Greatly expanded the time and space for technical applications.
3, high penetration rate. The average person can inoculate 3000-5000 units of material per day (8 hours). A production skilled worker who has been training for four months can successfully cultivate a single plant variety of 30,000-10,0000 pure seedlings per month. The requirements for the quality of personnel are highly adaptable, and the production technology is easy to popularize. Second, the speed of individual operations is much faster than that of tissue culture and conventional breeding. When reaching a certain scale, the investment ratio of production investment can reach 1:5-1. : 10 or more. It is very economizing on plant germplasm materials. It can inoculate hundreds of thousands of plants up to a million a day (this is an unimaginable thing for tissue culture around the world) and it is easy to rapidly propagate a wide range of seedlings in large areas. , It is easy to grow a large area of ​​seedlings to industrialize the scale of rapid propagation of various seedlings.
4. There are few operation steps and the production technology is simple. It is easy to master by special training and can be widely used in production. Suitable for large-scale planting seedlings. Ordinary staff can participate in the rapid production of all production operations, and the speed is very fast, the time of seedlings is short, which greatly saves the integrated production cost and improves the staff's production efficiency. With less one-time investment, low cost, and extremely high labor efficiency, labor costs are greatly saved and seedling production efficiency is improved.
5, technology operability, easy to industrialization. Each trained and qualified production technology backbone can produce and manage 3-20 million seedlings per month for different plants. Every person who is trained for more than 4 months can produce 30-100 million qualified seedlings per year. Create a very high economic efficiency. Many project bases with annual output of more than 10 million have already emerged in the industrialization development.
6, to obtain complete regeneration of plants faster, faster reproduction. The survival rate of nursery is as high as 85%--100%. The roots are well developed, generally 3-6 roots, and more than 30-40 roots. Rooting time is short, most plants. From the first day of inoculation, after 15 to 60 days of rooting, germination, obtaining complete regenerated plants, and hardening seedlings, the nursery can be planted. Realized efficient and rapid propagation. Each regenerated plant reproduces every 15 to 60 days, with a proliferation coefficient of 2-15 times per generation. With the original number of plants on the basis of the geometric number of highly efficient proliferation of significant features. This is similar to the advantages of rapid tissue culture in vitro culture.
7. Adopting an original set of effective technical systems and management experience, the TERNPC's technical indicators are composed of more than 200 unilateral or compound clones of liquid supplements for different plants.
8. The regenerated seedlings are strong, and the plants all show no mutation, early flowering results, and high yield. Many plants have blossomed in nursery land that year. Some flowers bloom 20-70 days from the date of inoculation, and the flowers are large and colorful. The flowering period is longer than that produced by other technical methods.
9. Mature seedlings, high purity, no pests, well-developed roots, root length more than 25 cm, root number 3-6, mature buds full, stem height 20-60 cm, planting production field survival rate of more than 90% .
10. The green seedlings directly inoculated in the nutrition bag (or tray) have high purity, no pests, growth of 3-5 leaf or more, full axillary buds, strong branching ability, well-developed root systems, high quality seedlings, and colonization production. The survival rate of the field reached more than 95%, and the growth rate exceeded the growth rate of the conventional cutting seedlings.
The plant non-tube high-efficiency rapid propagation technology (TERNPC) has driven the rapid development of a brand new rapid propagation pilot industry and has created a new revolution in the field of domestic and foreign economic plant development and utilization and vegetative propagation. It has a wide range of applications. It can be used in most plants that have been propagated using plant tissue culture stem tip rapid propagation and conventional cuttage propagation techniques, or plants that neither of the above two technologies are suitable for breeding; can be used for plant breeding or endangered economic plants. Widespread promotion and protection; can be used for asexual selection, purification and large-scale production and utilization of natural mutant plants, superior individuals and polyploid dominant plants; can be used for isolation and propagation of virus-free seedlings; can be used for introduction and diffusion of new varieties at home and abroad; available The most rapid propagation of various excellent transgenic plants and large-scale promotion as soon as possible.
Since plant non-tube high-efficient rapid propagation technology (TERNPC) was first developed at home and abroad, nearly 550 kinds of economic plants have been proposed in domestic production to achieve rapid propagation success. So far this technology has been used to promote the production of hundreds of millions of pure seedlings, and many economic plants have been used and produced for more than two decades. At present, the technology has been widely applied throughout the country; more than 30 Cologne technology affiliates and cooperative rapid propagation bases have been established nationwide. A number of associated companies have been identified as high-tech enterprises.
Plant non-tube high-efficient rapid propagation technology (TERNPC) has reached or exceeded the rapid propagation of plant in vitro comprehensive efficiency. In the history of asexuality and rapid propagation of human plants, it has universal significance for the rapid propagation of a variety of economic plants, and for the first time achieved the highest speed of production and reproduction with the lowest production cost, more economical and social benefits than any previous technology. And ecological benefits. The rapid propagation of many plant varieties undertaken by this technology has begun to play an increasingly important role in the country’s ecological environment construction, agricultural industrial restructuring, eco-city construction, and western development. The technology continues to be steadily promoted throughout the country and will become the largest seedling technology in vegetative propagation technology in the next 5 to 10 years. Experts believe that this innovative "plant cloning" new technology has reached the leading level at home and abroad and is an "atomic bomb" of biotechnology. It will bring a big change to China's and the world's economic rapid planting industry. Bring vitality to the motherland and the world's green ecological industry. With the progress of the industrialization of the technology and the continuous development of more successful new varieties, it will make even greater contributions to mankind.
The industrialization of the technique of efficiant and rapid non-test tube plant clones has broad market prospects. The contradiction between the supply and demand of seedlings production will be very prominent in a long period of time. Therefore, the promotion of plant non-tube high-efficiency and rapid propagation technology industrialization projects will be implemented in various places throughout the country as soon as possible, and will be implemented in order to accelerate the construction of ecological environment, eco-city construction, increase of forest coverage rate, western development, and adjustment of agricultural industry structure. contribution.
Plant non-tube high-efficient rapid propagation technology (TERNPC) is a green industrial project, and its social and ecological benefits are very prominent. As a high technology, it has unique characteristics of low investment, low risk, and high efficiency. The most suitable for industrial production, modern enterprise mechanism operation. The technology is high in technology and the market prospect is extremely broad. Industrialization promotion has high economic benefits. Only the supply of seedlings has a high potential for profit. According to the investment of 150-200 million yuan, production and supply of 10 million seedlings of different prices calculated, with an average total profit of more than 800 million. According to the investment of 750-1000 million yuan, production and supply of 50 million seedlings, the company's total annual profit can reach more than 8,000 million. According to the investment of 1500-2000 million yuan, the production and supply of 100 million seedlings will be calculated, and the company's annual profit will reach 160 million yuan. This kind of economic benefit is unmatched by general production companies. In the case of a certain amount of sales of seedlings, the selling price of seedlings directly determines the profitability of business operations. According to the production (sales) of 50 million seedlings, when the average price of a single plant is 1 yuan, the sales volume at the breakeven point is 964,000, and the sales volume is 9.64 million yuan, accounting for 0.9% of the planned sales volume, less than 1% . When the average price of a single plant reached 3 yuan, the sales amount was only 854,000 yuan, and the balance between profit and loss was reached. The balance point was only 0.6% and it was closer to zero. This situation fully proves: First, the price is indeed a more sensitive factor in the industrialization of this technology. But at the same time, it also shows that the competitive advantage of the price under this technical condition, from the price of 1-3 yuan per plant, to 3 yuan per plant, the risk is close to 0, down to 1 yuan/plant, and the risk is only 2.0%. The safety factor is high. Second, in this sense, we further explain the high-efficiency meaning of this technology and its great potential for industrialization.
At present, Li Changli, a researcher of non-tube high-efficiency and rapid propagation technology in China, has trained more than 360 people engaged in production technology and has prepared urgently needed talents for the industrialization of the technology. It is estimated that more than 2,500 production workers will be engaged in this technology next year. Among them, there are 13 people with relevant professional senior technical titles, 22 with intermediate professional titles, and all employees have high overall quality and strong technical strength. In addition, there are also a large number of management personnel with high quality and high management capacity in the joint ventures in the country, which are suitable for the industrialized operation of non-tube high-efficiency rapid propagation technology in plants. They have made important contributions to the promotion of the plant technique of efficient and rapid non-tube clones, complete modern management systems and production systems. All managers and technicians who have engaged in the industrialization of the technique of efficiant & rapid non-test tube plant clone have formed a consensus: "To clone seedlings, first clone the clone seedlings. People (clonic sprouts), including clone management backbones and technical backbones."
Cologne Industrial Co., Ltd. established its own website, Cologne Plant Network, as early as in 1997, and established an English and traditional Chinese version of the website. In 2003, it also established the Industrialization of Plant Propagation in Cologne, China. As the website becomes more and more perfect, network technology plays an important role in promoting rapid propagation technology.
Msct Scanner,Gemstone Detector,Vascular CT Scan,Conventional CT Scan
MinFound Medical Systems Co., Ltd , https://www.minfoundmed.com