Zhang Shuqiong hematocrit is an important hemorheological index. The increase of the results is not only related to cerebral infarction, but also to polycythemia. The decrease means anemia or blood loss, so the accuracy of the measurement is crucial. . Micro-high-speed centrifugation has long been introduced by the International Hematology Standards Committee (ICSH). However, so far, there are few manufacturers of micro-high-speed centrifuges in China, and some products have quality problems. One of the TGL-12B micro-high-speed centrifuges purchased in our room has a centrifugal speed of less than 12000r/min, and the sealing cement is detached or broken by capillary. To this end, we have adopted several trials and comparisons to reduce the rotational speed and extend the centrifugation time, overcoming the shortcomings of the instrument and achieving satisfactory results. 1 Materials and methods 1.1 Sample source Heparin anticoagulant specimens for hemorheology in outpatients and inpatients were used. 1.2 Measuring equipment (1) Instruments: Shanghai "Flying Pigeon" TCL-12B micro-high speed capillary centrifuge (digital display type, common type each), zui high speed 12000r/min, centrifugal radius 8.5cm,zui Large relative centrifugal force of 13600g, adjustable speed, horizontal rotary type, can be placed 24 capillaries, timing range 0 ~ 30min. (2) Glass capillary: produced by Huaxi Medical University Instrument Factory, with internal diameter of 0.9-1.1mm, wall thickness of 0.10-1.15mm, tube length of 120mm, and taking 55-60mm when used. (3) Others: soap, platy rubber mud, calculator, ruler. 1.3 Method Blood sample is mixed, the blood of the capillary wall of the blood sample is wiped off, and the end of the nozzle is sealed with glue and soap, and placed symmetrically in the flat disk slot of the micro centrifuge. The sealing end is in contact with the inner wall of the flat plate, the cover plate is covered, the screw is tightened, and the centrifugation is performed at a constant speed. 2 Results 2.1 Comparison of hematocrit measured at different time and speed. 24 samples were randomly centrifuged at different times and speeds on two centrifuges. The measurements were taken out at the same time (hematocrit = red blood cell volume / whole blood) Total volume × 100%). The mean ± standard deviation of each group was calculated, and the 12000r/min 5min group was used as the control to calculate the variance. 2.2 The centrifugation time and the rotational speed were consistent. The capillary volume values ​​measured by the capillary length were compared with 12 specimens on the same centrifuge. The centrifugation time and the rotational speed are the same, and the capillary length is not the same as the measured hematocrit results. The results are shown in Table 2. 3 Discussion Centrifuge is a tool for measuring hematocrit. The internationally recommended method for detecting hematocrit is micro-high-speed centrifugation, which has the advantages of low blood volume, rapid (12000r/min, 5min), and good reproducibility. At present, there are almost no supporting or qualified micro-high-speed centrifuge equipment for domestic manufacturers of blood rheology inspection instruments. There are only a handful of manufacturers that produce microcentrifuges separately. Shanghai Feige brand centrifuge is a well-known manufacturer specializing in the production of various centrifuges for many years. Our department has purchased the parameters of the two TGL-12B micro-high-speed centrifuges and the air test have met the setting requirements. However, in practical applications, we have encountered a common type. When the rotational speed reaches 8000r/rain or more, the microcapillary is almost powdery and cannot be measured. The digital display type is superior to the ordinary type, but if the continuous operation time is too long, the glue may be removed or the capillary may be broken. The reason may be that the temperature inside the machine is fast, the heat dissipation and the shock absorbing device are defective, and the quality of the centrifugal disk is related. These are issues that need to be discussed with the manufacturer. In order to overcome the above deficiencies, we used the digital display type 12000r/min centrifugation for 5min for the red blood cell pressure value as reference, and adopted the method of reducing the rotation speed and prolonging the centrifugation time to test different time and speed segments. It can be seen from Table 1 that the number of hematocrit values ​​of the digital display type 7000r/min 8min, 10000r/min 7min, and 12000r/min 5min is equal in each group, and occasional colloidal detachment and capillary rupture. Ordinary type 5000r/min 13min, 600 (O/min 10min, 7080r/min8min, the average of each group is equal to the digital display type 12000r/min 5mln. After the ordinary type 800Of/min 6min, the capillary is taken out and the result is undetectable. The results show that it is very important to choose a qualified centrifuge. The newly purchased centrifuge should be tested in real time to find the quality problem. When the centrifugal speed is less than 12000r/min (13600g), you can choose to reduce the rotation speed and extend the centrifugation time. In order to centrifuge until the hematocrit no longer sinks. Each laboratory should set its own reference range. (The normal reference range of our department: male 37% 47%, female 35% 43%) 10000r/min 7min, ordinary type 600 (O/min 10rain or 7080r/mln 7min, two instruments are rotated, no delay time, and can overcome the high speed of the machine, the temperature rise in the machine is fast, the capillary sealing glue is removed or the capillary The fracture phenomenon is accurate and reliable. Author unit: 610031 l~Jll Province Chengdu Third People's Hospital Research Laboratory
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