Elemental analysis is an important part of food analysis. The methods used for elemental analysis mainly include atomic absorption spectroscopy, inductively coupled plasma optical emission spectroscopy, inductively coupled plasma-mass spectrometry, atomic fluorescence, etc. Pre-experimental treatment. The preparation and pretreatment of the sample is to convert the sample into a physical state and a chemical state suitable for separation and measurement. It is related to the sensitivity, precision and accuracy of the analytical determination. The common methods of treatment are: dilution method, high temperature drying. Ashing method, low temperature dry ashing method, atmospheric pressure wet digestion method, high pressure wet digestion method, combustion method, hydrolysis method, microwave digestion method, and the like.
The experimental pre-processing method was established with reference to relevant data, and the elements of copper, manganese, zinc, iron, lead, chromium and cadmium were determined by flame atomic absorption method or graphite furnace atomic absorption method according to the mass of the element to be tested in the sample solution. In the determination of lead, chromium and cadmium by graphite furnace atomic absorption, ammonium dihydrogen phosphate was added as a matrix modifier, and the content of each element to be tested was determined according to the instrument operating procedure.
Experimental equipment: atomic absorption spectrophotometer (detection of trace heavy metal elements in food, medicine, and natural environment).
Sample Preparation: Weigh a certain amount of food sample into a dedicated digestion tube of the two-stage digestion apparatus, add 5 mL of nitric acid, and insert the digestion tube into the well of the digestion apparatus. Open the lower layer heating switch of the digestion device, set the heating temperature between 150~200 °C, and carry out sample digestion; after the sample digestion is complete (the solution is clear), open the upper layer heating switch of the digestion device, set the heating temperature to be between 100~200 °C, Remove the acid until a small amount of yellow transparent sample solution is left. After cooling, transfer to a 25 mL volumetric flask and bring up to volume. At the same time, do a blank sample experiment.
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